• USA Home
  • G5003 - α-Glucosidase from Saccharomyces cerevisiae

EMAIL THIS PAGE TO A FRIEND
G5003 Sigma

α-Glucosidase from Saccharomyces cerevisiae

Type I, lyophilized powder, ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)

Synonym: α-D-Glucosidase, α-D-Glucoside glucohydrolase, Maltase from yeast

Purchase

Properties

Related Categories α-Glucosidase, 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Amylases, α-Glucosidase, Starches (Amylose and Amylopectin) and Glycogen, Application Index,
type   Type I
form   lyophilized powder
contains   phosphate buffer salts and EDTA as balance
composition   Protein, ≥50%
foreign activity   β-Glucosidase, α−galactosidase and β−galactosidase ≤0.1%
storage temp.   −20°C
Gene Information   bakers yeast ... MAL12(853209), MAL32(852602)

Description

Analysis Note

Protein determined by biuret.

Application

For the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters

α-glucosidase is used for the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. It was also used in the measurement of glycosidase inhibition.

Biochem/physiol Actions

Hydrolysis of terminal, non-reducing 1→4-linked D-glucose residues with release of D-glucose.

α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

100 units in glass bottle

Sold on basis of p-nitrophenyl α-D-glucoside units.

1000 units in poly bottle

Unit Definition

One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37 °C.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

inhibitor

Product #

Description

Add to Cart

B8299 N-Butyldeoxynojirimycin, film (dried in situ)
D1542 1,4-Dideoxy-1,4-imino-D-arabinitol hydrochloride, enzyme inhibitor
C3784 Castanospermine, ≥94% (GC), BioUltra, from Castanospermum australe seeds

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

substrate

Product #

Description

Add to Cart

N1377 4-Nitrophenyl α-D-glucopyranoside, ≥99%
M9766 4-Methylumbelliferyl α-D-glucopyranoside, α-glucosidase substrate

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?