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G5025 Sigma

β-Galactosidase-biotin labeled from Escherichia coli

lyophilized powder, 350-1200 units/mg protein

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
form   lyophilized powder
composition   Protein, ~75% E1%/280
extent of labeling   2-5 mol d-biotin per mol protein
storage temp.   −20°C

Description

Packaging

Package size based on protein content

Preparation Note

Biotinylated using biotin–maleimide (B 1267) which incorporates an extended spacer.

Unit Definition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside per min at pH 7.3 at 37 °C.

Physical form

Lyophilized powder containing Tris-acetate, DTT, MgCl2, and isopropyl β-D-thiogalactopyranoside

Application

β-Galactosidase was used as a control antigen in the selection of human antibody fragments by phage display.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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