Granulocyte-Macrophage Colony-Stimulating Factor human recombinant, expressed in E. coli, suitable for cell culture

Properties

Related Categories	Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines as adjuvants, Cytokines, Growth Factors and Chemokines for Stem Cell Differentiation, Cytokines, Growth Factors and Hormones, Cytokines, Growth Factors, Chemokines for Stem Cell Biology, Cytokines, Growth Factors, and Chemokines for Stem Cell Expansion, GM-CSF, General Stem Cell Biology, Growth Factors, Growth Factors and Cytokines, Hematopoietic Cytokines, Hematopoietic Stem Cell Biology, Hematopoietic Stem Cell Cytokine and Growth Factors, Mesenchymal Stem Cell Biology, Mesenchymal Stem Cell Biology Growth Factors, Reagents and Supplements, Stem Cell Biology, Stem Cell Differentiation, Stem Cell Expansion, Vaccine Adjuvants, Vaccine Production More...
recombinant	expressed in E. coli
assay	≥98% (SDS-PAGE and HPLC)
form	lyophilized powder
potency	≤0.1 ng/mL EC₅₀
mol wt	mol wt 14.6 kDa
suitability	suitable for cell culture
storage temp.	−20°C

Description

Frequently Asked Questions

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Analysis Note

The proliferative activity of human GM-CSF is tested in culture using human TF-1 cells.¹

Biochem/physiol Actions

Granulocyte-macrophage colony stimulating factor is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.