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  • G5038 - Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in rabbit

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G5038 Sigma

Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-GAD 65

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Neurobiology,
species reactivity   human, pig, rat, mouse, monkey
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using tissue sections of rat pancreas.
  microarray: suitable
  western blot: 1:4,000 using rat brain extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the N-terminal region of human GAD 65 (amino acids 5-22). The sequence is highly conserved in rat and pig GAD 65 (single amino acid substitution) and mouse and monkey GAD 65, but is not found in GAD 67.

General description

GAD is predominantly expressed in the central nervous system (CNS) and pancreatic islets. It is also expressed in testis, oviduct and ovary. GAD exists in two isofoms, GAD 65 and GAD 67. GAD65 is targeted to membranes and nerve endings.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Reacts specifically with GAD 65 (65 kDa).

Application

Anti-Glutamic Acid Decarboxylase 65 antibody produced in rabbit is suitable for use as a primary antibody at a working dilution of 1:1000 in fluorescent immunohistochemistry of stomach and jejunum sections of mice. It is suitable for immunoblotting at a working dilution of 1: 4000 using a rat brain extract. It is also suitable for indirect peroxidase immunohistochemical staining of formalin-fixed, paraffin-embedded sections of rat pancreatic islet cells at a working dilution of 1:1000.

Features and Benefits

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Biochem/physiol Actions

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L-glutamate to γ-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain, and a putative paracrine signal molecule in pancreatic islets. GAD65 is an ampiphilic, membrane-anchored protein, (585 amino acid residues) and is encoded on human chromosome 10. It has been identified as an autoantigen in insulin-dependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS) and may serve as a marker in the early stages of IDDM. GAD 65 synthesizes transmitter GABA for vesicular release.

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