G5080 Sigma

Sephadex® G-50




Frequently Asked Questions

Frequently Asked Questions are available for this Product.


10, 50, 100, 500 g in poly bottle


Sephadex® G-50 is a gel filtration medium used in affinity chromatography, protein chromatrography and gel filtration chromatography. Sephadex® G-50 has been used to investigate effects from snake venom, isolate lectins from various species and to demonstrate the positive influence of 20% ethanol on proteinase desorption.

Legal Information

Sephadex is a registered trademark of GE Healthcare

Price and Availability

Amplified Detection
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option:
What does "G-50" mean in Sephadex®  G-50, Product G5080?
"G" stands for gel.  "50" refers to the water regain of the gel, in this instance, 5.0 g water per g dry gel.
What is the best buffer to use with Sephadex®  G-50, Product G5080?
Sephadex® G-50 is very flexible in the buffers that can be used with it.  Essentially any common aqueous buffer can be used with Sephadex® G-50.  It is advised to filter buffers through a 0.22 micron filter before use.
How stable is Sephadex® G-50, Product G5080, after swelling?
Sephadex® is stable in water, salt solutions, organic and denaturing solvents.  If necessary, the gel can be removed from the column and sterilized by autoclaving at 120 deg C, pH 7. The pH stability is limited to low strengths and short times at extremes of pH 2-10, particularly in the acid range.  After any washes using solutions at extremes of acidic or basic pH, the Sephadex®  should be washed and reequilibrated with buffer at neutral pH as promptly as possible.
How can I clean the Sephadex®  G-50, Product G5080, after use?
To clean the gel, you can wash the gel with 2 column volumes (CV) of 0.2 M NaOH, outside of the column because the gel will swell in basic pH, or in a solution of non-ionic detergent.  Then you can rinse with water and re-equilibrate with 2-3 CV of buffer. If the resin is to be stored (longer than ~24 hours), equilibrate the resin with buffer containing an antimicrobial such as 20% ethanol or 0.02% sodium azide.
What kind of separation can I expect from Sephadex®  G-50, Product G5080?
As a general rule of thumb, Sephadex® G-50 is appropriate for the separation of molecules of ~30 kDa from molecules ~1.5 kDa
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles


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Peer-Reviewed Papers


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GE17-0042-02 Sephadex® G-50, Fine, GE Healthcare, pack of 500 g G5080-500G is replaced by GE17-0042-02

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