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G5149 Sigma

Gly-Gln monohydrate

≥97% (HPLC)

Synonym: Glycyl-L-glutamine

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Miscellaneous Neuropeptides, Neuropeptides, Peptides and Proteins,
InChI Key   KLFWVRQACSYLOH-WCCKRBBISA-N
assay   ≥97% (HPLC)
storage temp.   −20°C

Description

Amino Acid Sequence

Gly-Gln

Application

Heat-stable substitute for glutamine in mammalian cell culture media.

Packaging

1, 5 g in glass bottle

Biochem/physiol Actions

Gly-Gln monohydrate has been used for the preparation of glutamine solution in HTF (human tubal fluid) medium to assess the accumulation of ammonium in commercially available culture media and protein supplements for in vitro development of human pre-implantation embryos, when stored at 2–8°C and incubated at 37°C.

General description

Glycyl-glutamine (gly-gln) is a dipeptide that is used as a substitute for glutamine in cell culture. Higher cell yields are obtained when this dipeptide is used instead of glutamine, where gly-gln affects substrate utilization and metabolism. Cell growth is higher as the concentrations of this dipeptide remain high, due to lower peptidase affinity.

Price and Availability


Amplified Detection

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

L-Glutamine in Cell Culture

Importance and uses of glutamine in serum-free eucaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Keywords: Alternative energy, Cell culture, Culture media, Deaminations, Metabolism, Oxidations, Transaminations, Vitamins

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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