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  • G5163 - Anti-Glutamic Acid Decarboxylase 65/67 antibody produced in rabbit

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G5163 Sigma

Anti-Glutamic Acid Decarboxylase 65/67 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-GAD 65/67

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Neurobiology,
species reactivity   feline, rat, mouse, pig, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using tissue sections of rat pancreas
  microarray: suitable
  western blot: 1:10,000 using rat brain extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 65-67 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminal region of human GAD 67 (amino acids 579-594). The sequence is identical in human GAD 65 (amino acids 570-585), in rat, mouse, and pig GAD 65, in rat, pig, and cat GAD 67, and is highly conserved in GAD 67 of mouse origin (single amino acid substitution).

General description

Glutamic acid decarboxylase (GAD) is the key enzyme that catalyses the conversion of L-glutamate to γ-aminobutyric acid (GABA). GABA is a principal inhibitory neurotransmitter and a paracrine signal molecule in pancreatic islet cells. GAD is highly expressed in GABAergic neurons and pancreatic β cells. It is transiently expressed in non-GABAergic embryonic and adult neurons, indicating a role in development. It is also present in testis, oviduct, and ovary
GAD exists as two isoforms, GAD 65 and GAD 67 (molecular masses of 65 and 67 kDa, respectively) that are encoded by two different genes. GAD65 is an ampiphilic, membrane anchored protein, (585 amino acid residues) and is encoded on human chromosome 10. GAD 67 is a cytoplasmic protein (594 amino acid residues) and is encoded on chromosome 2. There is 64% amino acid idENTITY_TYPE_CODE. The two isoforms appear to have distinct intraneuronal distribution in the brain.
GAD 65 has been identified as an autoantigen in insulin dependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Reacts specifically with GAD 65 and GAD 67 isoforms from brain.

Reacts specifically with GAD 65 and GAD 67 isoforms in extracts of rat brain and rat pancreas.

Application

Anti-GAD 65/67 may be used for the detection and localization of GAD 65/67 isoforms by immunofluorescence and immunohistochemistry in rat retinal cells, human subthalamus neurons, and pancreatic β-cells of rat. The minimum working dilution that may be used is 1:10000. Detection by immunoblotting may be possible in brain cells of monkey, human, rat and gecko. at a minimum working dilution of 1:1000.

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