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G5251 Sigma

DL-Glyceraldehyde 3-phosphate solution

45-55 mg/mL in H2O

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Description

Packaging

500 mg in glass bottle

Application

The level of Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) activity in HEK 293 cells was aβeβed by measuring the reduction of NAD+ by DL-Glyceraldehyde 3-phosphate.

Biochem/physiol Actions

Glyceraldehyde 3-phosphate is an intermediate in several metabolic pathways, including glycolysis and gluconeogenesis. It is bactericidal and a potent inhibitor of growth in E. coli.

Other Notes

Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. G5251.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 1760 8 / PGII
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Aerobic Glycolysis and the Warburg Effect

The Warburg effect is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer ce...
Keywords: Aerobic, Biofiles, Cancer, Cell proliferation, Clinical, Glycolysis, PAGE, Phosphorylations, Support, Transcription

Calvin Cycle

The Calvin cycle is a more complex process used by algae, plants and photosynthetic bacteria to convert carbon dioxide and water into carbohydrate and molecular oxygen with the aid of sunlight. The l...
BioFiles 2007, 2.6, 27.
Keywords: Aldol condensation, Biofiles, Catalysis, Condensations, Isomerizations, Phosphorylations, Reductions

Glycolysis Overview

Carbohydrates synthesized during photosynthesis act as the main storage molecules of solar energy. When ingested, complex carbohydrates are enzymatically hydrolyzed to monosaccharides, such as starch...
BioFiles 2007, 2.6, 4.
Keywords: Aldol reaction, Biofiles, Dehydration reaction, Glycolysis, Isomerizations, Metabolism, Metabolites, Metabolomics, Phosphorylations, Photosynthesis

Monosaccharide Biosynthesis

Each of the monosaccharides has its own interesting metabolism. The pathway by which a monosaccharide is made, is not the reverse of its degradation pathway (see Figure 4).
BioFiles 2007, 2.6, 21.
Keywords: Addition reactions, Biofiles, Carboxylations, Catalysis, Condensations, Decarboxylations, Degradations, Metabolic Pathways, Metabolism, Metabolites, Phosphorylations, Reductions

Pentose Phosphate Pathway

While glucose metabolism by glycolysis occurs where energy is needed quickly, e.g. in brain and muscle cells, a second pathway for glucose metabolism, called pentose phosphate pathway, operates in ti...
BioFiles 2007, 2.6, 25.
Keywords: Catalysis, Glycolysis, Metabolism, Oxidations, Pentose phosphate pathway

Peer-Reviewed Papers
15

References

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