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G5507 Sigma

Galactosyltransferase from bovine milk

lyophilized powder

Synonym: Lactose Synthase, UDP-galactose:D-glucose 4-β-galactosyltransferase

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Properties

Related Categories 2.4.x.x Glycosyltransferases, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
form   lyophilized powder
activity   3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)
composition   Protein, 35-65% modified Warburg-Christian
impurities   ≤10 % protein α-lactalbumin
storage temp.   −20°C

Description

Linkage

This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.

Packaging

25 units in glass bottle

5, 10 units in serum bottle

Unit Definition

One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

Physical form

Lyophilized powder containing Tris, EDTA, and (NH4)2SO4

Application

Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.

Biochem/physiol Actions

Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Glycosyltransferases

Glycosyltransferases are specific for the type of linkage (α or β), and the linkage position of the glycoside bond formed [e.g. α(1→3) or β(1→4)]. Glycosyltransferases were initially considered to be...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Catalysis, Cell signaling, Enzyme activity, Glycosylations, Pharmaceutical, Type

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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