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Gene Knockout Protocols

  •  ISBN-10 0-89603-572-7

  •  ISBN-13 978-0-89603-572-0

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Related Categories Books and Software, Genetics Books, Labware, Molecular Biology Books
publication info   M.J. Tymms and I. Kola, ed., Humana Press, 2001, 350 pp., hard cover
mfr. no.   Humana

Description

General description

Highly skilled investigators in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for the analysis of the mouse phenotype. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. State-of-the-art and highly practical, it constitutes an invaluable source of readily reproducible techniques for genetic researchers today.

Table of Contents

Overview: Gene Knockouts
Isolation and Maintenance of Primate Embryonic Stem Cells
Gene Targeting in ES Cells,
Manipulating Mouse Embryonic Stem Cells.
Gene Targeting in a Centralized Facility,
The LoxP/CRE System and Genome Modification,
Creation and Use of a Cre Recombinase Transgenic Database
Choice of Mouse Strains for Gene Targeting
Isolation, Microinjection, and Transfer of Mouse Blastocysts
Aggregation Chimeras: Combining ES Cells, Diploid and Tetraploid Embryos
How to Study Pathologic Phenotypes of Knockout Mice
Analysis of Hematopoietic Phenotypes in Knockout Mouse Models.
Isolation of Embryonic Fibroblasts and Their Use in the In Vitro Characterization of Gene Function
Influence of Genetic Background on Knockout Mouse Phenotypes
Lineage Allocation During Early Embryogenesis: Mapping of the Neural Primordia and Application to the Analysis of Mouse Mutants
Generation of Double-Knockout Embryonic Stem Cells
In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes
Embryonic Stem Cells in the Study of Hematopoiesis
Interferon-Inducible ES Cell Expression Systems,
Transgenic Studies in the Mouse: Improving the Technology Towards a Conditional Temporal and Spatial Approach,
In Vivo Libraries of Large Insert Transgenic Mice for Genetic Mapping
Epigenetic Effects on Transgene Expression
Positional-Candidate Cloning of Genes from Mouse Mutants
Genetically Engineered Mice: Husbandry and Resources
Embryo Cryopreservation for Transgenic Mouse Lines
Index.

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