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G6046 Sigma

Anti-G3BP antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Ras-GTPase-activating protein SH3-domain binding protein 1, Anti-G3BP1, Anti-GAP SH3 domain-binding protein, Anti-HDH-VIII, Anti-human DNA helicase VIII

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Oxidative Stress Proteins,
species reactivity   human, mouse (predicted), rat (predicted)
application(s)   indirect immunofluorescence: 2-5 μg/mL using human HeLa cells
  western blot: 0.1-0.2 μg/mL using whole extract of human U-2-OS cells
clone   polyclonal
concentration   ~1.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~68 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... G3BP1(10146)
mouse ... G3bp1(27041)
rat ... G3bp1(171092)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acid residues 234-247 of human G3BP, conjugated to KLH via an added cysteine. The corresponding sequence differs by 4 amino acids in rat and mouse.

General description

G3BP is a RNA-binding protein that contains a RNA binding domain, the RRM-type domain, at the carboxyl-terminal. The N-terminal domain is homologous to nuclear transporter factor 2 (NTF2) and the central domain is rich in acidic residues. The RRM domain is involved in the binding of G3BP to specific RNA sequences. This helps G3BP to function as a CA dinucleotide-specific endoribonuclease. G3BP′s subcellular localization and protein-protein interactions are mediated by the phosphorylation of G3BP at Ser149, near NTF2-like domain. G3BP is found in abundance in many kinds of malignant tumors such as lung, colon, gastric, and breast cancer.

Antigen Background

G3BP encodes one of the DNA-unwinding enzymes which prefers partially unwound 3′-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

In immunoblotting assays, it is highly recommended to dilute the antibody in 5% NFDM (w/v).

Application

Anti-G3BP antibody produced in rabbit is suitable for use as a primary antibody in immunofluorescence analysis of capsid or cellular marker proteins of SINV (Sindbis virus)-infected Vero cells. It is also useful in the immunostaining of vero cells transfected with CHIKV replicon RNA for localization of G3BP. It is suitable for use in immunoblotting at a working concentration of 0.1–0.2μg/mL using a whole extract of human U-2-OS cells. It is also suitable for use in immunofluorescence using human HeLa cells at a working concentration of 2–5μg/mL.

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Biochem/physiol Actions

G3BP (Ras-GTPase-activating protein SH3 domain binding protein 1) is a endoribonuclease that is phosphorylation-dependent and specific to single strand. It cleaves between cytosine and adenine (CA). It binds to the SH3 domain of RasGAP. It forms a link between RasGAP-mediated signaling pathway and RNA turnover. G3BP is required for the assembly of stress granules (SGs), which are involved in the regulation of mRNA metabolism during stress. It may be used as a cytoplasmic SG marker.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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G8046 Anti-phospho-G3BP (pSer149) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

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