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G6160 Sigma

Monoclonal Anti-β-COP antibody produced in mouse

clone maD, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to COP Vesicles, Antibodies to Cell and Organelle Proteins,
species reactivity   monkey, rat, human, hamster
application(s)   indirect immunofluorescence: 1:80 using cultured Chinese hamster ovary (CHO) cells
  microarray: suitable
  western blot: 1:1,000 using a preparation of stacked Golgi membranes from rat liver
clone   maD, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... COPB1(1315)
rat ... Copb1(114023)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide D1 of β-COP (a.a. 701-715) conjugated to KLH

General description

The coatomer (approx. 550kDa) consists of proteins designated α-, β-, γ-, and δ-COP, together with substoichiometric amounts of several other proteins.

Specificity

The antibody recognizes an epitope in the β-COP protein (110 kDa) and stains the periphery of the Golgi complex using immunocytochemical techniques.

Application

Monoclonal Anti-β-COP antibody produced in mouse is suitable for use as a primary antibody in immunoblot:
• analysis at a working dilution of 1:1000 using subcellular proteins from rat PC12 (pheochromocytoma) cells
• analysis of gradient fractions of cerebral microvessels to confirm the separation of plasma membrane lipid raft domains from intracellular membranous components
• detection of the Golgi marker protein β-COP in exosome-enriched extracellular microvesicles (eMV) preparations from untreated HeLa cells

It is suitable for immunostaining of β-coatomer, that is used as a intracellular, Golgi protein marker :
• to confirm that CD14 staining is localized to the cell surface of HAEC
• for examining the localization of Meltrin β in the Golgi apparatus and its vicinity in neurons prepared from developing dorsal root ganglia of mouse embryos
• in NB4 and NB4-LR1 cells to examine the colocalization of PKA regulatory subunits
• in CHO cells to examine colocalization of GFP-Rab24

It is suitable for use in cell-surface ELISA of human aortic endothelial cells (HAEC)

It is also suitable for western blot analysis at a working dilution of 1:1000 using a preparation of stacked Golgi membranes from rat liver, for indirect immunofluorescence at a working dilution of 1:80 using cultured Chinese hamster ovary (CHO) cells and for microarray.

Features and Benefits

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Biochem/physiol Actions

COPs (coatomer proteins) contain adaptin-like, complex (8) and are transiently attached to the vesicles involved in transport within the Golgi complex and possibly between the rough ER and Golgi complex. β-COP has a molar mass of 110kDa and its primary structure is homologous to the β-adaptin component of clathrin-coated vesicles.

Price and Availability


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