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  • G6171 - Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal

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G6171 Sigma

Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal

clone G-A-5, purified from hybridoma cell culture

Synonym: Anti-GFAP

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   rat, pig, human
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  indirect immunofluorescence: 2.5-5 μg/mL using alcohol-fixed sections of rat brain/cerebellum.
  western blot: suitable
clone   G-A-5, monoclonal
concentration   ~1.0 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~50 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GFAP(2670)
mouse ... Gfap(14580)
pig ... GFAP(396562)
rat ... Gfap(24387)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

purified GFAP from pig spinal cord.

General description

Glial fibrillary acidic protein or GFAP is a type III intermediate filament protein that is found in the astrocytes. GFAP assembly has been implicated in mitotic remodeling of glial structures. Genetic mutations in GFAP have been associated with Alexander disease
Monoclonal Anti-Glial Fibrillary Acidic Protein is specific for GFAP in rats, pigs and humans. The antibody does not bind to vimentin. The product stains glial cells, astrocytes Bergmann gliomas and other glial tumors by indirect immunofluorescence (using alcohol-fixed or frozen sections).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Monoclonal Anti-Glial Fibrillary Acidic Protein antibody is suitable for use in western blot and indirect immunofluorescence 2.5-5 μg/mL using alcohol-fixed sections of rat brain/cerebellum). The antibody can also be used for immunoblot (approx. 50 kDa), immunohistochemistry (in paraformaldehyde-fixed, frozen rabbit brain sections) and immunocytochemistry.

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