• USA Home
  • G6171 - Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal

EMAIL THIS PAGE TO A FRIEND
G6171 Sigma

Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal

clone G-A-5, purified from hybridoma cell culture

Synonym: Anti-GFAP

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   pig, rat, human
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  indirect immunofluorescence: 2.5-5 μg/mL using alcohol-fixed sections of rat brain/cerebellum.
  western blot: suitable
clone   G-A-5, monoclonal
concentration   ~1.0 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~50 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GFAP(2670)
mouse ... Gfap(14580)
pig ... GFAP(396562)
rat ... Gfap(24387)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

purified GFAP from pig spinal cord.

General description

Glial fibrillary acidic protein or GFAP is a type III intermediate filament protein that is found in the astrocytes. GFAP assembly has been implicated in mitotic remodeling of glial structures. Genetic mutations in GFAP have been associated with Alexander disease
Monoclonal Anti-Glial Fibrillary Acidic Protein is specific for GFAP in rats, pigs and humans. The antibody does not bind to vimentin. The product stains glial cells, astrocytes Bergmann gliomas and other glial tumors by indirect immunofluorescence (using alcohol-fixed or frozen sections).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Monoclonal Anti-Glial Fibrillary Acidic Protein antibody is suitable for use in western blot and indirect immunofluorescence 2.5-5 μg/mL using alcohol-fixed sections of rat brain/cerebellum). The antibody can also be used for immunoblot (approx. 50 kDa), immunohistochemistry (in paraformaldehyde-fixed, frozen rabbit brain sections) and immunocytochemistry.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Price and Availability


Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
Protocols & Articles

Articles

Chondroitinase for Neural Regeneration Research

The spinal cord is the information highway system for the brain. And axons are the road that allows information data to be transferred. So, when a traumatic spinal cord injury (SCI) occurs and axons ...
Keywords: Catalysis, Cell culture, Degradations, High performance liquid chromatography, Immunofluorescence, PAGE

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

Related Products

antibody

Product #

Description

Add to Cart

G4546 Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
G9269 Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
C9205 Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP)−Cy3 antibody produced in mouse, clone G-A-5, purified from hybridoma cell culture
G3893 Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse, clone G-A-5, ascites fluid

related product

Product #

Description

Add to Cart

06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?