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G6278 Sigma

Glycerokinase from Escherichia coli

300-600 units/mL, ammonium sulfate suspension

Synonym: ATP:glycerol 3-phosphotransferase, Glycerol Kinase

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Description

Unit Definition

One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.

Physical form

Suspension in 3.1 M (NH4)2SO4 pH 7.3, with 1% BSA and 2% trehalose

Application

Glycerol kinase (glpK) was used to study the effects of pain controlling neuropeptides on human fat cell lipolysis.

Biochem/physiol Actions

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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