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G6282 Sigma

Monoclonal Anti-β-Galactosidase antibody produced in mouse

clone GAL-40, ascites fluid

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Properties

Related Categories β-Galactosidase Detection, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Pancreatic Stem Cells,
species reactivity   bacteria
application(s)   dot blot: suitable using soluble β-galactosidase
  immunocytochemistry: suitable
  western blot: 1:1,000 using denatured-reduced Escherichia coli β-galactosidase
clone   GAL-40, monoclonal
antibody form   ascites fluid
isotype   IgM
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   Escherichia coli ... lacZ(1036228)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

β-D-galactosidase from E. coli

General description

Eukaryotic genes are usually cloned into E.coli β-galactosidase (lacZ) gene that results in a fusion hybrid of the desired gene product with the β-galactosidase enzyme . As the amount of the desired gene product in these cloned vector systems corresponds to the amount of β-galactosidase, enzyme assays for β-galactosidase can be utilized to detect the required gene product . Monoclonal anti-β-galactosidase antibodies are specific for β-galactosidase in E.coli and enable the analysis of β-galactosidase fusion proteins encoded by cloned vector systems.

Application

Monoclonal anti-β-galactosidase antibodies may be used in dot blot (using soluble β-galactosidase), western blot (at a dilution of 1:1,000, using denatured-reduced Escherichia coli β-galactosidase) and immunocytochemistry. This antibody may also be used for immunochemical assays such as ELISA, immunoblot and immunohistochemistry by using the stepwise procedure or by the preparation of β-galactosidase anti-β galactosidase (BBAG) complex. Additionally, this product is suitable for the detection and purification of recombinant fusion proteins encoded by cloning vectors containing the β-galactosidase gene. Monoclonal anti-β-galactosidase antibody is also used for the immunoenzymatic staining of bone marrow and blood smears, and tissue sections.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
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