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G6539 Sigma

Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse

clone GFP-20, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Recombinant Protein Analysis, Antibodies to Fluorescent Labels, Antibodies to Peptide and Protein Fusion Tags,
biological source   mouse
antibody form   ascites fluid
clone   GFP-20, monoclonal
mol wt   antigen mol wt 27 kDa
contains   15 mM sodium azide
application(s)   dot blot: suitable
  indirect ELISA: suitable
  western blot: 1:2,000 using purified recombinant GFP preparation
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Immunogen

GFP tagged fusion protein.

GFP tagged fusion protein

Application

Monoclonal Anti-Green Fluorescent Protein (GFP) antibody is suitable for use in western blot and ChIP assays.

General description

The Green Fluorescent Protein (GFP) is derived from the jellyfish Aequorea Victoria and is often used as a gene expression marker. Thus, antibodies to GFP can facilitate the detection and analysis of GFP labeled nucleic acids and proteins. Monoclonal Anti-Green Fluorescent Protein (GFP) antibody is specific for GFP and GFP-labeled biomolecules.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

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G1544 Anti-GFP, N-terminal antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
G6795 Monoclonal Anti-Green Fluorescent Protein (GFP), N-terminal antibody produced in mouse, ~2 mg/mL, clone GSN24, purified immunoglobulin, buffered aqueous solution
G1546 Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse, clone GSN149, purified immunoglobulin, buffered aqueous solution
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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