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  • G6541 - Monoclonal Anti-GRP1 antibody produced in mouse

G6541 Sigma

Monoclonal Anti-GRP1 antibody produced in mouse

~2 mg/mL, clone CYT3-10, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-ARNO3, Anti-Cytohesin-3



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to ARF and Related Proteins, Antibodies to G Proteins,
species reactivity   rat, human
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 2-4 μg/mL using whole extract of cultured human NRK (normal rat kidney) cells
clone   CYT3-10, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 45 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CYTH3(9265)
rat ... Cyth3(116693)
biological source   mouse
conjugate   unconjugated



recombinant human GRP1 (general receptor for phosphoinositides-1).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Monoclonal Anti-GRP1 antibody produced in mouse is suitable for immunocytochemistry, indirect ELISA and microarray. It is also suitable for western blot analysis at a working concentration of 2-4μg/mL using whole extract of cultured human NRK (normal rat kidney) cells.

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Biochem/physiol Actions

GRP1 gene encodes a protein belonging to the PSCD (pleckstrin homology, Sec7 and coiled-coil domains) family. The members of this family contain an N-terminal coiled-coil motif that is involved in homodimerization, a central Sec7 domain with guanine-nucleotide exchange protein (GEP) activity, and a C-terminal pleckstrin homology (PH) domain that interacts with phospholipids and facilitates association of PSCDs with membranes. This protein is responsible for the control of Golgi structure and function via the activation of ARF1 on Golgi membrane. It is also involved in the regulation of ARF6 functions.

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