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G6751 Sigma

α-Glycerophosphate Dehydrogenase from rabbit muscle

Type I, ammonium sulfate suspension, 100-300 units/mg protein

Synonym: sn-Glycerol-3-phosphate:NAD+ 2-oxidoreductase, sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
type   Type I
form   ammonium sulfate suspension
foreign activity   Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
  Triosephosphate isomerase ≤0.02%
storage temp.   2-8°C

Description

Analysis Note

Protein determined by biuret.

Preparation Note

Prepared by modification of the method of Fondy.

Unit Definition

One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4 and 0.1 g/L EDTA solution, pH 6.0

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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