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G6916 Sigma

Anti-GADD153 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-CHOP-10

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies for Embryonic Stem Cells,
species reactivity   human
application(s)   immunocytochemistry: 5-10 μg/mL using Hela human cell
  microarray: suitable
  western blot (chemiluminescent): 1:200-1:400 using whole cell extract of HEK-293 over-expressing GADD 153.
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 29 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DDIT3(1649)
rat ... Ddit3(29467)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

peptide corresponding to human GADD153/CHOP10 sequence (amino acids 153-169) with N-terminal lysine added, conjugated to keyhole limpet hemocyanin (KLH). The corresponding sequence in mouse and rat differs by 2 amino acids.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Application

Anti-GADD153 antibody produced in rabbit is suitable as a primary antibody in western blot analysis of:
• nuclear and cytoplasmic fractions extracted from rat insulinoma (INS) cells to study CHOP Expression in response to ER stress
• whole cell lysates prepared from ER stress induced rat pancreatic tissues
• whole cell lysates prepared from ER stress induced pancreatic insulin-producing cells

It is suitable for immunocytochemistry at a working concentration of 5-10μg/mL using Hela human cell and microarray. It is also suitable for western blotting at a working dilution of 1:200-1:400 using whole cell extract of HEK-293 over-expressing GADD 153.

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Biochem/physiol Actions

GADD153/CHOP10 (Growth Arrest and DNA Damage Inducible Protein 153, C/EBP Homology Protein) is a leucine zipper transcription factor that lacks a DNA binding site. The protein homodimerizes with members of the CCAAT/enhancer binding protein (C/EBP) family of transcription factors during adipogenesis. Many signals induce the expression of CHOP10. Some of these signals include endoplasmic reticulum stress, hyperosmosis, growth arrest, DNA damage, calcium onophore, nutrient deprivation, hypoxia and hyperoxia. GADD153 heterodimerizes with C/EBPb and inhibits its binding to DNA sequences. Expresssion of this gene induces growth arrest in fibroblasts, inhibits adipocyte differentiation, and is associated with apoptosis in vitro. GADD153 binds to several nuclear proteins as well as the FTE/S3a ribosomal protein and enhances erythroid differentiation. It negatively regulates ATF3, a stress-inducible transcriptional repressor.

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