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G7017 Sigma

β-Glucuronidase from Helix pomatia

Type HP-2, aqueous solution, ≥100,000 units/mL

Synonym: β-D-Glucuronide glucuronosohydrolase

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Description

Linkage

Similar to G0876, but produced by Sigma.

Packaging

25 mL in glass bottle

Quality

Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Some preparations have been reported to contain acid phosphatase activity.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

Application

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSn) analysis, to study the structures of degradation products of baicalin.

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail.
Optimal pH:
• Glucuronidase activity: 4.5 to 5.0
• Sulfatase activity: ~6.2
Inhibitors: D-glucuronic acid, D-galacturonic acid, D-glucaro-1, 4-lactone

General description

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Price and Availability

Suggested Laboratory Gloves


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Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS. Here we evaluate the hydrolysis efficiency of β-gluc...
Jim Blasberg
Harkewal Singh, Kevin Ray Sigma-Aldrich, St. Louis, MO
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites

Protocols

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

To standardize a procedure for Enzymatic Assay of b-Glucuronidase1 at Sigma-Aldrich St. Louis.
Keywords: Enzymology

Related Content

Analytical Enzyme Beta Glucuronidase

Sigma® β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from urine1,2, plasma3,4, and other fluids5 prior to analysis by enzyme immunoassay, mass spectrometry, gas chr...
Keywords: Bacterial conjugations, Cancer, Chromatography, Gas chromatography, High performance liquid chromatography, Immunoassay, Mass spectrometry, PAGE, Purification

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
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