β-Glucuronidase from Helix pomatia
Type HP-2, aqueous solution, ≥100,000 units/mL
DOWNLOAD MSDS (PDF)Synonym:
β-
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CAS Number 9001-45-0
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MDL number MFCD00130629
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Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
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Properties
| Related Categories | β-Glucuronidase, 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Analytical and Industrial Enzymes, Application Index, |
| Quality Level | PREMIUM |
| type | Type HP-2 |
| form | aqueous solution |
| secondary activity | ≤7,500 units/mL sulfatase |
| storage temp. | 2-8°C |
Description
Linkage
Similar to G0876, but produced by Sigma.
Quality
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Some preparations have been reported to contain acid phosphatase activity.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Physical form
Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
Application
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.1
Biochem/physiol Actions
β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.2,3,4
β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail.
Optimal pH:
• Glucuronidase activity: 4.5 to 5.0
• Sulfatase activity: ~6.2
Inhibitors:
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Price and Availability
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