|Related Categories||β-Glucuronidase, 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Analytical and Industrial Enzymes, Application Index,|
|secondary activity||≤7,500 units/mL sulfatase|
Similar to G0876, but produced by Sigma.
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Some preparations have been reported to contain acid phosphatase activity.
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.1
β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.2,3,4
β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail.
• Glucuronidase activity: 4.5 to 5.0
• Sulfatase activity: ~6.2
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
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Type B-1, ≥1,000,000 units/g solid
Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)
Type H-2, aqueous solution, ≥85,000 units/mL
Type HP-2S, aqueous solution, ≥90,000 units/mL
Type H-1, partially purified powder, ≥300,000 units/g solid
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1. OBJECTIVE To standardize a procedure for Enzymatic Assay of b-Glucuronidase1 at Sigma-Aldrich St. Louis.
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
4. Catalytic mechanisms of enymatic glycosyl transfer M.L. Sinnott Chem. Rev. 90, 1171-1202, (1990)
Cross-talk between tetraspanin CD9 and transmembrane adaptor protein non-T cell activation linker (NTAL) in mast cell activation and chemotaxis. Hálová I, Dráberová L, Bambousková M, et al. J. Biol. Chem. 288(14), 9801-14, (2013)
FGF23 is a novel regulator of intracellular calcium and cardiac contractility in addition to cardiac hypertrophy. Touchberry CD, Green TM, Tchikrizov V, et al. Am. J. Physiol. Endocrinol. Metab. 304(8), E863-73, (2013)
Antitumor efficacy of the heparanase inhibitor SST0001 alone and in combination with antiangiogenic agents in the treatment of human pediatric sarcoma models. Cassinelli G, Lanzi C, Tortoreto M, et al. Biochem. Pharmacol. 85(10), 1424-32, (2013)
Endothelial heparanase regulates heart metabolism by stimulating lipoprotein lipase secretion from cardiomyocytes. Wang Y, Zhang D, Chiu AP, et al. Arterioscler. Thromb. Vasc. Biol. 33(5), 894-902, (2013)
Targeting of heparanase-modified syndecan-1 by prosecretory mitogen lacritin requires conserved core GAGAL plus heparan and chondroitin sulfate as a novel hybrid binding site that enhances selectivity. Zhang Y, Wang N, Raab RW, et al. J. Biol. Chem. 288(17), 12090-101, (2013)
[Construction of a lentiviral RNA interference system targeting heparanase based on miR30 and its silencing effect]. Liu X, Fang H, Zhu D, et al. Zhejiang Da Xue Xue Bao. Yi Xue Ban 42(1), 67-74, (2013)
Rapid determination of N-acetyl-4-aminophenol (paracetamol) in urine by tandem mass spectrometry coupled with on-line clean-up by two dimensional turbulent flow/reversed phase liquid chromatography. Modick H, Schütze A, Pälmke C, et al. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 925, 33-9, (2013)
α-Klotho and kidney function decline: an important step forward in understanding the link between mineral metabolism and kidney disease progression. Gutiérrez OM and Ix JH Am. J. Kidney Dis. 61(6), 855-7, (2013)
The comparative ability of beta-glucuronidase preparations (liver, Escherichia coli, Helix pomatia, and Patella vulgata) to hydrolyze certain steroid glucosiduronic acids. WAKABAYASHI M and FISHMAN WH J. Biol. Chem. 236, 996-1001, (1961)
Weirich, G.F., et al. Arch. Insect Biochem. Physiol. 3, 109, (1986)
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|P4411||pH test strips, 7.0-14.0 pH, resolution: 0.5 pH unit|
|G0876||β-Glucuronidase from Helix pomatia, Type H-2, aqueous solution, ≥85,000 units/mL|
|I0880||Isoxsuprine hydrochloride, analytical standard, for drug analysis|
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|H1254||8-Hydroxyquinoline glucuronide, ≥95%, powder|
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