|Related Categories||β-Glucuronidase, 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Analytical and Industrial Enzymes, Application Index,|
|secondary activity||≤7,500 units/mL sulfatase|
Similar to G0876, but produced by Sigma.
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Some preparations have been reported to contain acid phosphatase activity.
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.1
β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.2,3,4
β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail.
• Glucuronidase activity: 4.5 to 5.0
• Sulfatase activity: ~6.2
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
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Type B-1, ≥1,000,000 units/g solid
Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)
Type H-2, aqueous solution, ≥85,000 units/mL
Type HP-2S, aqueous solution, ≥90,000 units/mL
Type H-1, partially purified powder, ≥300,000 units/g solid
Certificate of Analysis
Certificate of Origin
1. OBJECTIVE To standardize a procedure for Enzymatic Assay of b-Glucuronidase1 at Sigma-Aldrich St. Louis.
4. Catalytic mechanisms of enymatic glycosyl transfer M.L. Sinnott Chem. Rev. 90, 1171-1202, (1990)
Weirich, G.F., et al. Arch. Insect Biochem. Physiol. 3, 109, (1986)
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|P4411||pH test strips, 7.0-14.0 pH, resolution: 0.5 pH unit|
|G0876||β-Glucuronidase from Helix pomatia, Type H-2, aqueous solution, ≥85,000 units/mL|
|I0880||Isoxsuprine hydrochloride, analytical standard, for drug analysis|
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|H1254||8-Hydroxyquinoline glucuronide, ≥95%, powder|
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