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G7153 Sigma

Guanidine hydrochloride

BioXtra, ≥99.5% (titration)

Synonym: Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride

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Properties

Related Categories Biochemicals and Reagents, Cell Lysis and Protein Extraction Reagents, Chaotropic Agents, Core Bioreagents, Denaturation,
InChI Key   PJJJBBJSCAKJQF-UHFFFAOYSA-N
Quality Level   ELITE
product line   BioXtra
assay   ≥99.5% (titration)
impurities   Insoluble matter, passes filter test
ign. residue   ≤0.05%
loss   ≤0.05% loss on drying, HV, 20°C
pH   4.5-6.0 (6 M in H2O)
mp   180-185 °C(lit.)
solubility   H2O: 6 M clear, colorless
anion traces   sulfate (SO42-): ≤0.005%
cation traces   Al: ≤0.0005%
  As: ≤0.0001%
  Ba: ≤0.0005%
  Bi: ≤0.0005%
  Ca: ≤0.001%
  Cd: ≤0.0005%
  Co: ≤0.0005%
  Cr: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Li: ≤0.0005%
  Mg: ≤0.0005%
  Mn: ≤0.0005%
  Mo: ≤0.0005%
  Na: ≤0.005%
  Ni: ≤0.0005%
  Pb: ≤0.0005%
  Sr: ≤0.0005%
  Zn: ≤0.0005%
absorption   A6M/260, H2O ≤0.1
  A6M/280, H2O ≤0.05

Description

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Price and Availability


Ultrafree Centrifugal Filter Units
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
MF4300000
Protocols & Articles
Peer-Reviewed Papers
15

References

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