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G7295 Sigma

Anti-GM130 (C-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-GM130 (Golgi Matrix Protein of 130 kDa), Anti-Golgi Autoantigen, Anti-Golgi Matrix Protein GM 130

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology,
species reactivity   mouse, rat, human
application(s)   immunoprecipitation: 2-4 μg using Hela and 3T3 cell extracts.
  indirect immunofluorescence: 0.2-0.4 μg/mL using rat NRK cells
  western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of rat NRK cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~130 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GOLGA2(2801)
mouse ... Golga2(99412)
rat ... Golga2(64528)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminal region of human GM130 with an N-terminal added lysine, conjugated to KLH. The corresponding sequence is identical in rat and mouse GM130.

General description

GM130 is a peripheral membrane protein that is bound to the Golgi complex. GM130 regulates a broad range of cellular functions such as cell movement, cell cycle, Golgi body assembly, glycosylation, and cell polarization . Anti-GM130 (C-terminal) antibody is specific for GM130 in humans, mice and rats. The immunizing peptide specifically inhibits the detection of the GM130 band by immunoblotting. The antibody may detect additional bands in some cell and tissue extracts.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7,.4, containing 15 mM sodium azide.

Application

Anti-GM130 (C-terminal) antibody can be used in immunoblotting (approx. 130 kDa), indirect immunofluorescence (0.2-0.4 μg/mL using rat NRK cells), and immunoprecipitation (2-4 μg using Hela and 3T3 cell extracts). The antibody can also be used for chemiluminescent western blot (0.1-0.2 μg/mL using whole extract of rat NRK cells).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Organelle Isolation | BioFiles Volume 6, Number 5 — Centrifugation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, a...
Mark Frei
BioFiles v6 n5, 22–25
Keywords: Capture ELISA, Cell culture, Centrifugation, Diffusion, Direct immunofluorescence, Dot blot, Enzyme-linked immunosorbent assay, Flow cytometry, Immunoassay, Immunocytochemistry, Immunoelectrophoresis, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Indirect ELISA, Individual protein Immunoprecipitation, Microarray Analysis, Microscopy, Radioimmunoassay, Scanning electron microscopy, Western blot

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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