Anti-GM130 (C-terminal) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
DOWNLOAD MSDS (PDF)Synonym: Anti-GM130 (Golgi Matrix Protein of 130 kDa), Anti-Golgi Autoantigen, Anti-Golgi Matrix Protein GM 130
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MDL number MFCD01633397
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Popular Documents: Datasheet (PDF)
Properties
| Related Categories |
Alphabetical Index,
Antibodies,
Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology,
Antibodies against Proteins/Bioactives/Marke |
| species reactivity | mouse, rat, human |
| application(s) | immunoprecipitation: 2-4 μg using Hela and 3T3 cell extracts. |
| indirect immunofluorescence: 0.2-0.4 μg/mL using rat NRK cells | |
| western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of rat NRK cells | |
| clone | polyclonal |
| antibody form | affinity isolated antibody |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~130 kDa |
| shipped in | dry ice |
| storage temp. | −20°C |
| Gene Information |
human ... GOLGA2(2801) mouse ... Golga2(99412) rat ... Golga2(64528) |
Description
Immunogen
synthetic peptide corresponding to amino acid residues 968-983 of human GM130 with an N-terminal added lysine, conjugated to KLH. The corresponding sequence is identical in rat and mouse GM130.
General description
GM130 is a peripheral membrane protein that is bound to the Golgi complex. GM130 regulates a broad range of cellular functions such as cell movement, cell cycle, Golgi body assembly, glycosylation, and cell polarization 1. Anti-GM130 (C-terminal) antibody is specific for GM130 in humans, mice and rats. The immunizing peptide specifically inhibits the detection of the GM130 band by immunoblotting. The antibody may detect additional bands in some cell and tissue extracts.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7,.4, containing 15 mM sodium azide.
Application
Anti-GM130 (C-terminal) antibody can be used in immunoblotting (approx. 130 kDa), indirect immunofluorescence (0.2-0.4 μg/mL using rat NRK cells), and immunoprecipitation (2-4 μg using Hela and 3T3 cell extracts). The antibody can also be used for chemiluminescent western blot (0.1-0.2 μg/mL using whole extract of rat NRK cells).
Price and Availability
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