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G7402 Sigma

Anti-Rabbit IgG (whole molecule)–Gold antibody produced in goat

affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)

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Properties

Related Categories Alphabetical Index, Antibodies, Gold, Primary Antibodies, RAB-RAD,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   aqueous glycerol suspension
availability   not available in Canada (at this time; for questions or status updates, please email us at antibody.canada@sial.com)
application(s)   dot blot: suitable
  western blot: suitable
particle size   10 nm (colloidal gold)
conjugate   gold conjugate
storage temp.   2-8°C

Description

Immunogen

Rabbit IgG purified from normal rabbit serum.

Packaging

0.4 mL in glass insert

2 mL in glass bottle

Physical form

Colloidal suspension in Tris buffered saline, pH 8.2, with 30% glycerol (v/v), 1% bovine serum albumin (w/v), and 15 mM sodium azide.

Application

Anti-rabbit IgG (whole molecule)-gold antibody (diluted 1: 15) can be used in immunoelectron microscopy. It can also be used in western blotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immuno-electron microscopy (1 paper)
Immunohistochemistry (1 paper)

General description

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. Anti-rabbit IgG (whole molecule)-gold antibody can be used as secondary antibody (diluted 1:50 in 0.01 M PBS containing 1% BSA and 5% NGS) in EM immunolabeling of GABA and glutamate by postembedding procedure. It can also be used in a modified dot blot assay to determine binding activity. Goat anti-rabbit IgG (whole molecule)-gold antibody reacts specifically with all goat immunoglobulins.

Immunoglobulins (Igs) are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs can be useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Gold antibody is specific for rabbit IgG.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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