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G7509 Sigma

GABase from Pseudomonas fluorescens

lyophilized powder, Protein ≥30 % by biuret

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

10, 25 units in poly bottle

Unit Definition

One unit will convert 1.0 μmole of γ-aminobutyric acid (GABA) to succinic semialdehyde and then to succinate per min with a stoichiometric reduction of 1.0 μmole of NADP+ at pH 8.6 at 25 °C.

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Application

GABase from Pseudomonas fluorescens has been used for GABase assay to quantify the extracellular and intracellular concentration of GABA (γ-aminobutyric acid).

Biochem/physiol Actions

GABase is the main enzyme used for the enzymatic analysis of GABA (γ-aminobutyric acid).

General description

GABase is a commercially available mixture composed of γ-aminobutyric acid aminotransferase (GABGT) and succinic semialdehyde dehydrogenase (SSDH) obtained from Pseudomonas fluorescens.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
How do I solubilize Product No. G7509, GABase from Pseudomonas fluorescens?
Sigma recommends to dissolve the product with 75 mM phosphate buffer, pH 7.2, containing 25% (v/v) glycerol to a concentration of 2-5 units per mL. Keep the solution in an ice bath while using and store solution frozen. This solution may be stored frozen and thawed several times with only slight loss of activity.
What activity assay is used for Product No. G7509, GABase from Pseudomonas fluorescens?
The assay may be found at the following URL:   http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Enzyme_Assay/gabase.Par.0001.File.tmp/gabase.pdf.
What is the shelf life of the Product No.G7509, GABase from Pseudomonas fluorescens?
Sigma recommends retesting the product 3 years after its release date. The retest date will appear on the lot specific Certificate of Analysis for the product.
Are there any literature references that cite use of the Product No. G7509, GABase from Pseudomonas fluorescens?
The product is cited in the reference Sulaimana, Saba A.J., et al., J of Enz Inhib and Med Chem. 18(4), 297-301 (2003).
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Protocols & Articles
Peer-Reviewed Papers
15

References

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