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  • G7650 - Monoclonal Anti-Glycophorin A,B (α,δ) antibody produced in mouse

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G7650 Sigma

Monoclonal Anti-Glycophorin A,B (α,δ) antibody produced in mouse

clone E3, purified from hybridoma cell culture

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cellular Antigens,
species reactivity   human
application(s)   agglutination assay: 1:400
  flow cytometry: suitable using bone marrow nucleated cells
  indirect immunofluorescence: suitable using bone marrow nucleated cells
  western blot: 0.5-1 μg/mL using extracts of red blood cell ghosts2
clone   E3, monoclonal
antibody form   purified immunoglobulin
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GYPA(2993), GYPB(2994)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human thymus

Physical form

Solution in 0.1 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

By immunoblotting, the antibody localizes specifically the α, δ, αδ, α2, δ2, α2δ and α3 bands in extracts of human red blood cell ghosts. The antibody (also cited as clone no. 6H5) binds to 12% of bone marrow nucleated cells in smears and tissue preparations using immunofluorescent microscopy or flow cytometry.

Application

Monoclonal Anti-Glycophorin A,B (α, δ) antibody produced in mouse is suitable for:
• immunoblot analysis to monitor the shaving completion of human RBC membrane protein components on the cytoplasmic surface, the extracellular surface, and the erythrocyte cytoskeleton.
• immunoprecipitation of glycophorin A/B from erythrocyte membrane lysate.
The antibody is suitable for localization of glycophorins A and B using:
• immunoblot analysis at a working concentration of 0.5-1μg/mL with extracts of red blood cell ghosts
• indirect immunofluorescence and flow cytometry with bone marrow nucleated cells.
It is also suitable for use in agglutination assay at a working dilution of 1:400.

Biochem/physiol Actions

Glycophorins (GP) are sialic acid-rich polypeptides (sialoglycoproteins) that are part of the erythrocyte membrane. They are denoted α, β, γ, δ based on the decreasing molar mass. GPA and GPB are the major constituents of the red cells. They may be present as single polypeptides (α and δ), as stable homodimers (α2 and δ2) and heterodimers (αδ). Depending upon the amino acid residues at positions 1 and 5, GPA carries blood group M or N. Based on the aminoacid substitution at residue 29, GPB carries blood group N and S activity. GPA is associated exclusively with erythroid cells. It is expressed in pronormoblasts and later erythroid cells.

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