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G7663 Sigma

Gelatin blocking buffer

for Western blotting, powder blend

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Description

Application

Suitable for use as a non-specific blocking agent for Western blots.

General description

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

Packaging

1 L in poly bottle

Reconstitution

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Protocols

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

Product Description Reagents Reagents Required but not Provided Preparation Instructions Storage/Stability Procedure Troubleshooting Guide Materials References
Keywords: Detergents, Enzyme activity, Immobilization, Mass spectrometry, PAGE, Southern blot, Western blot

Peer-Reviewed Papers
15

References

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