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G7765 Sigma

Gelatin from cold water fish skin

40-50% in H2O

Synonym: Gelatine, Teleostean gelatin

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Used as a blocking agent in immunochemistry.

Packaging

1 L in poly bottle

250 mL in poly bottle

Biochem/physiol Actions

Gelatin was used for coating cell culture to improve the attachment of cells. It was used as a blocking reagent in Western blotting, ELISA, and immunohistochemistry. Gelatin can also be used as a component of media for species differentiation in bacteriology. Gelatin is a biocompatible polymer and has been used as a delivery vehicle for the release of bioactive compounds and in the generation of scaffolds for engineering applications.
Gelatin was used in the murine lethality assay which is used to examine the ability of SMAbs, either alone or in combination, to neutralize the effects of BoNT/A1 in vivo.
Recommended for use as a cell culture substratum at 0.1-0.2 mg/cm2 or 5-10 μl/cm2. Optimal concentration depends on cell type as well as the application or research objectives.
Gelatin was used to study the ultrastructural distribution of the α7 nicotinic acetylcholine receptor subunit in rat hippocampus. It was also used in a study to test soybean protein concentrate as a protein source for turbot Scophthalmus maximus L.

General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is Product G7765, Gelatin from cold water fish skin, soluble in?      
Gelatin is soluble in glycerol and acetic acid, and more soluble in hot water than in cold water.  Additional information on gelatin can be found at our website: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/productinformationsheet2/g7765pis.Par.0001.File.tmp/g7765pis.pdf
What is the solution stability of Product G7765, Gelatin from cold water fish skin?
The specification for shelf life is 2 years for unopened bottles stored at 2-8°C.  Each lot is assigned a retest date; this date is given on the Certificate of Analysis avialable from our website.
What is the difference between type A and type B gelatin?
Type A gelatin is derived from acid-cured tissue whereas Type B gelatin is derived from lime-cured tissue. Type A gelatin has about 78-80 millimoles of free carboxyl groups per 100 g of protein and a pI of 7.9-9.0. Type B has 100-115 millimoles of free carboxyl groups per 100 g of protein and a pI of 4.7-5.2.  Additional information on gelatin can be found at our website: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/productinformationsheet2/g7765pis.Par.0001.File.tmp/g7765pis.pdf
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the gel point of Product G7765, Gelatin from cold water fish skin?
The gel point of Product G7765, Gelatin from cold water fish skin is 5-10°C. The product will be a gel when storing the product at 2-8°C.
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Protocols & Articles

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*Materials Science Product Management Team, MilliporeSigma, Milwaukee, WI.
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