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G7765 Sigma

Gelatin from cold water fish skin

40-50% in H2O

Synonym: Gelatine, Teleostean gelatin

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Used as a blocking agent in immunochemistry.

Packaging

1 L in poly bottle

250 mL in poly bottle

Biochem/physiol Actions

Gelatin was used for coating cell culture to improve the attachment of cells. It was used as a blocking reagent in Western blotting, ELISA, and immunohistochemistry. Gelatin can also be used as a component of media for species differentiation in bacteriology. Gelatin is a biocompatible polymer and has been used as a delivery vehicle for the release of bioactive compounds and in the generation of scaffolds for engineering applications.
Gelatin was used in the murine lethality assay which is used to examine the ability of SMAbs, either alone or in combination, to neutralize the effects of BoNT/A1 in vivo.
Recommended for use as a cell culture substratum at 0.1-0.2 mg/cm2 or 5-10 μl/cm2. Optimal concentration depends on cell type as well as the application or research objectives.
Gelatin was used to study the ultrastructural distribution of the α7 nicotinic acetylcholine receptor subunit in rat hippocampus. It was also used in a study to test soybean protein concentrate as a protein source for turbot Scophthalmus maximus L.

General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

3D Printing of Carbon Fiber-Reinforced Composites

3D printing is a type of additive manufacturing that can be used to rapidly fabricate components with highly customizable geometries, most typically using a layer-by-layer fabrication process. 3D pri...
Zhenyu Bo* (Ph.D Candidate at Northwestern University) and Jia Choi*, PhD, Product Manager


*Materials Science Product Management Team, MilliporeSigma, Milwaukee, WI.
Keywords: Deposition, Nanomaterials, Nanotubes

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

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