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G8271 Sigma

β-Glucuronidase from Escherichia coli

Preweighed vials of G7646, vial of ≥1000 units

Synonym: β-D-Glucuronide glucuronosohydrolase

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Description

Quality

Does not contain added buffer.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

Application

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Biochem/physiol Actions

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

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Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
LZ8990000
Protocols & Articles

Related Content

Analytical Enzyme Beta Glucuronidase

Sigma® β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from urine1,2, plasma3,4, and other fluids5 prior to analysis by enzyme immunoassay, mass spectrometry, gas chr...
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Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
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Peer-Reviewed Papers
15

References

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G7646 β-Glucuronidase from Escherichia coli, Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)

substrate

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73677 4-Nitrophenyl β-D-glucuronide, ≥99.0% (TLC)
B8174 5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt, tablet
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