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G8289 Sigma

Glucose-6-phosphate dehydrogenase from torula yeast

ammonium sulfate suspension, 300-600 units/mg protein (biuret)

Synonym: G-6-P-DH

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Carbohydrate Metabolism, Cell Biology,
form   ammonium sulfate suspension
foreign activity   6-phosphogluconic dehydrogenase, hexokinase, phosphoglucomutase, phosphoglucose isomerase, or NADPH oxidase ≤0.05%
storage temp.   2-8°C

Description

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Physical form

Suspension in 2.6 M (NH4)2SO4 solution, pH 7.5

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Protocols

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To standardize a procedure for the enzymatic determination of Glucose-6-Phosphate Dehydrogenase.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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G7877 Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae), Type VII, ammonium sulfate suspension, ≥200 units/mg protein

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