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G8386 Sigma

Glutathione S-Transferase from rat liver

lyophilized powder, ≥15 units/mg protein

Synonym: Glutathione S-alkenetransferase, Glutathione S-alkyltransferase, Glutathione S-aralkyltransferase, Glutathione S-aryltransferase, Glutathione S-epoxidetransferase, RX: Glutathione R-transferase

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Properties

Related Categories 2.5.x.x Alkyl or aryl groups other than methyl, 2.x.x.x Transferases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder
composition   Protein, ~75%
storage temp.   −20°C
Gene Information   rat ... Gsta3(24421), Gstcd(310855)

Description

Analysis Note

Protein determined by biuret.
Purified and assayed by a modification of the method of Simons and Vander Jagt.
Enzymatic activities are based on the conjugation of reduced glutathione with a second substrate. The individual proteins generally have activity with more than one class of substrate.

Unit Definition

One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at 25°C.

Physical form

Lyophilized powder containing Tris, phosphate salts, reduced glutathione and EDTA

Biochem/physiol Actions

Glutathione S-transferases are a family of proteins that catalyze the conjugation of reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

WGK Germany 
3
Protocols & Articles

Articles

HPLC Analysis of Glutathione S-Transferase on TSKgel® G3000SWXL

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Glutathione S-Transferase on TSKgel® G3000SWXL
Keywords: Chromatography, Enzyme activity, High performance liquid chromatography

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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