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G8529 Sigma

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, lyophilized powder, 550-1,100 units/mg protein (biuret)

Synonym: G-6-P-DH

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Carbohydrate Metabolism, Cell Biology,
Quality Level   PREMIUM
recombinant   expressed in E. coli
form   lyophilized powder
composition   Protein, 10-40% biuret
Featured Industry   Agriculture
foreign activity   creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

200, 1000 units in glass bottle

2000, 10000 units in poly bottle

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Physical form

Lyophilized powder containing Ficoll and Tris buffer salts

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
For Product No. G8529, Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides, can the Ficoll present in the product be removed?
Ficoll is added to the enzyme preparation as a stabilizer. The enzyme may become unstable if the Ficoll is removed (i.e., through a dialysis step).
In which solvent can Product No. G8529, Glucose- 6-phosphate Dehydrogenase from Leuconostoc mesenteroides, be dissolved?
The product can be dissolved at 1 mg/ml in 5 mM glycine buffer, pH 8, (with or without 0.1% BSA) or in deionized water at 1 mg/ml (buffer is preferable)
Can solutions of Product No. G8529, Glucose-6-phosphate Dehydrogenase, recombinant from Leuconostoc mesenteroides, be stored?
Sigma has not evaluated the solution stability of this enzyme.  Perhaps solutions in buffer may be aliquotted and stored frozen at 20 deg. C for about 1-2 months.   Sigma offers the enzyme in an ammonium sulfate suspension, product G8404, for solution storage at 2-8 deg. C.
What are the differences between Product No. G8529, Glucose-6-phosphate Dehydrogenase and Product No. G5885, Glucose-6-phosphate Dehydrogenase?
Product No. G8529 is a recombinant protein from Leuconostoc mesenteroides, expressed in Escherichia coli.  Product No. G5885 is a non-recombinant protein from Leuconostoc mesenteroides.  In addition, there may be some differences in the specifications of each, located at our website: http://www.sigmaaldrich.com/catalog/DataSheetPage.do?brandKey=SIGMA&symbol=G8529 and http://www.sigmaaldrich.com/catalog/DataSheetPage.do?brandKey=SIGMA&symbol=G5885.  Both products are part of our Premium EQP (SAFC Enhanced Quality Program), http://www.safcglobal.com/safc-global/en-us/home/eqp.html.
What is the molecular weight of Product No. G8529, Glucose-6-phosphate Dehydrogenase?
Sigma has not determined the molecular weight of this protein, but a value of 55,000 daltons has been reported for the monomer molecular weight (the enzyme from Leuconostoc mesenteroides is reportedly a dimer protein) (Biochem. Biophys. Res. Comm. 59, 894 (1974)).
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Protocols & Articles

Protocols

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To standardize a procedure for the enzymatic determination of Glucose-6-Phosphate Dehydrogenase.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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