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G8642 Sigma

Glutathione S-Transferase from human placenta

lyophilized powder, 25-125 units/mg protein

Synonym: GST, Glutathione S-alkenetransferase, Glutathione S-alkyltransferase, Glutathione S-aralkyltransferase, Glutathione S-aryltransferase, Glutathione S-epoxidetransferase, RX: Glutathione R-transferase

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Properties

Related Categories 2.5.x.x Alkyl or aryl groups other than methyl, 2.x.x.x Transferases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder
composition   Protein, ~40% biuret
storage temp.   −20°C
Gene Information   human ... GSTM1(2944), GSTP1(2950)

Description

Analysis Note

Protein determined by biuret.
Purified and assayed by a modification of the method of Simons and Vander Jagt.
Enzymatic activities are based on the conjugation of reduced glutathione with a second substrate. The individual proteins generally have activity with more than one class of substrate.

Packaging

1, 5, 10 mg in glass bottle

Unit Definition

One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at 25°C.

Physical form

Lyophilized powder containing Tris buffer salts, reduced glutathione and EDTA.

Application

Glutathione S-Transferase from human placenta has been used for the immunization of rabbits for the production of specific anti-π-GST antibodies, to determine whether total anti-π-GST antibodies are more specific and reactive as compared to anti-peptide antibodies.

Biochem/physiol Actions

Glutathione S-transferase (GST) catalyzes conjugation reactions during phase II metabolism. This enzyme also confers protection against catabolic products of peroxidized lipid and oxidized DNA, and ROS (reactive oxygen species) or electrophiles.

Glutathione S-transferases are a family of proteins that catalyze the conjugation of reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers.

General description

Glutathione S-transferase (GST) is a major detoxification enzyme, and exists as multiple cytoplasmic and membrane-bound isozymes. Cytoplasmic isoforms of GST are encoded by five genes, namely α, γ, μ, σ and π. α, μ and π are the most abundant forms in mammals. GSTπ is the isoform of this enzyme found in placenta and is composed of 209 amino acids.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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