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G8898 Sigma

Glycine

for electrophoresis, ≥99%

Synonym: Aminoacetic acid, Aminoethanoic acid, Glycocoll

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Properties

Related Categories Amino Acids, Buffers, Buffers and Reagents, Buffers, Reagents, & Stains, Core Bioreagents,
grade   for electrophoresis
InChI Key   DHMQDGOQFOQNFH-UHFFFAOYSA-N
assay   ≥99%
form   powder
impurities   ≤0.01% Insoluble matter
pKa (25 °C)   2.35
mp   240 °C (dec.)(lit.)
solubility   H2O: soluble200 mg/mL, clear, colorless to faintly yellow
anion traces   chloride (Cl-): ≤70 ppm
cation traces   heavy metals (as Pb): ≤20 ppm
absorption   A1M/280 ≤0.15
Gene Information   rat ... Grin2a(24409)

Description

Analysis Note

Tested for use in electrode buffers for PAGE and in transfer buffers for Western blots.

Biochem/physiol Actions

Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Packaging

1 kg in poly bottle

500 g in poly bottle

Application

• Glycine has been used for the preparation of gelatin composites scaffold.
• It has been used in the elution buffer to extract protein using HiTrap protein G column.
• It has been used in the SDS-sample, running and transfer buffers prepared for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and western blotting.

General description

Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking gel. It has advantage of low mobility, hydrophobicity and it does not associate with proteins. Glycine is a component of Tris-glycine and Tris-glycine-SDS (sodium dodecyl sulfate) running buffers for polyacrylamide gel electrophoresis. Glycine is also a component of Towbin′s transfer buffer for Western blots.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
MB7600000
Protocols & Articles

Articles

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Peer-Reviewed Papers
15

References

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