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G9144 Sigma

Anti-Gliadin (Wheat) antibody produced in rabbit

fractionated antiserum, buffered aqueous solution

Synonym: Anti-Gliadin (Wheat)

Properties

Related Categories Alphabetical Index, Antibodies, GD-GL, Miscellaneous, Plant Biology,
species reactivity   wheat
application(s)   dot blot: 1:1,500
  indirect ELISA: 1:5,000
clone   polyclonal
antibody form   fractionated antiserum
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

native and heat-treated wheat gliadin

General description

Gliadin is a protein found in wheat and other cereals. The T cells recognize the gliadin epitopes and activate the innate immune response similar to the response to pathogens. This response is more intense in coeliac disease wherein the gut-derived T cells produced in response to gliadin induce damage to the small intestine. Therefore, patients affected by coeliac disease or gluten-sensitivity should avoid food that produce gliadin
Rabbit Anti-Gliadin shows specificity for native wheat gliadin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Anti-Gliadin (Wheat) antibody may be used for ELISA at a working antibody dilution of 1:2000. The antibody was used in dot blot and ELISA using human sera. Anti-gliadin was also used for dot blot of pooled sera at a working dilution of 1:3000.

Biochem/physiol Actions

Gliadin is a class of glutamine containing, alcohol soluble proteins separable from wheat and rye glutens known as the prolamins. These proteins are associated with the harmful effects of celiac disease and gluten sensitive enteropathy in humans, causing characteristic changes in the small intestinal mucosa. In such cases, strict compliance to a gluten-free diet is usually recommended.

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