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G9152 Sigma

Anti-Galactocerebroside antibody produced in rabbit

whole antiserum

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology,
species reactivity   human
application(s)   dot blot: 1:50 using galactocerebroside I and II
clone   polyclonal
antibody form   whole antiserum
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GALC(2581)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

Galactocerebrosides (GalC) I and II conjugated to KLH

Specificity

The antibody localizes oligodendroglia in cultured cells. It may be used for studying changes in the organization of oligodendroglial membrane sheets in culture. Specificity of the product is determined by dot blotting using galactocerebroside as the antigen.

Application

Anti-Galactocerebroside antibody produced in rabbit is suitable:
• for dot-blot immunoassay and western blot analysis of mouse brain homogenates.
• as a primary antibody at a working dilution of 1:50 for immunostaining of muscle tissue of Rana pipiens frogs
• as a primary antibody at a working dilution of 1:500 in immunofluorescence and immunohistochemistry using primary human astroglia
The antibody is useful for labeling myelin sheaths, oligodendrocytes and Schwann cells and for staining cultured cells, cell suspensions, frozen tissue sections and perfusion-fixed tissues. It is suitable for dot blot immunoassay at a working dilution of 1:50 using galactocerebroside type I and II.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence-cell culture cells (1 paper)

Biochem/physiol Actions

Galactocerebroside is the major galactosphingolipid of myelin. It is expressed in the CNS in cell membranes of oligodendrocytes. It is also expressed in Schwann cells in peripheral nerves. Galactocerebroside is found in serum and cerebral spinal fluid of patients with demyelinating diseases like multiple sclerosis.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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