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G9264 Sigma

Monoclonal Anti-Growth Associated Protein-43 antibody produced in mouse

clone GAP-7B10, ascites fluid

Synonym: Anti-GAP-43

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Neurobiology, Antibodies to Neural Proteins, GR-GZ,
species reactivity   hamster, mouse, snake, human, rat, feline, chicken
application(s)   immunohistochemistry: suitable
  western blot: 1:2,000 using newborn rat brain extract
clone   GAP-7B10, monoclonal
antibody form   ascites fluid
isotype   IgG2a
mol wt   antigen mol wt 46 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GAP43(2596)
mouse ... Gap43(14432)
rat ... Gap43(29423)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

GAP-43 from neonatal rat forebrain membranes.

Specificity

Recognizes an epitope present on both kinase C phosphorylated and dephosphorylated forms of GAP-43.

Application

Monoclonal Anti-Growth Associated Protein-43 antibody produced in mouse is suitable for:
• immunostaining of tissue sections from the prefrontal cortex and hippocampus of rat pups to recognize an epitope present on kinase C domain in the N terminal of GAP-43 protein
• immunohistochemistry at a working dilution of 1:3000 using sections from fetal and adult brains of mice
• immunofluorescence at a working dilution of 1:4000 using 8μm sections of mice brain
• western blotting using hippocampal lysates from rats
It is also suitable for immunoblotting at a working dilution of 1:2000 using newborn rat brain extract. The antibody is useful in the study of the role and function of GAP-43 during axonogenesis and in the adult nervous system.

Biochem/physiol Actions

The neural-specific Growth-Associated Protein-43 (GAP-43) is an intracellular phosphoprotein found exclusively in the peripheral and central nervous systems. The axonal growth cones that are involved in the functions of motility and pathfinding during axonogenesis and regeneration contain GAP-43. Phosphorylation of GAP-43 is associated with exocytosis, depolarization and treatment of growth cones with nerve growth factor (NGF).

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