• USA Home
  • G9282 - Monoclonal Anti-Glutamate antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
G9282 Sigma

Monoclonal Anti-Glutamate antibody produced in mouse

clone GLU-4, ascites fluid

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neurotransmission, Antibodies to Glutamate Receptors,
biological source   mouse
antibody form   ascites fluid
clone   GLU-4, monoclonal
contains   15 mM sodium azide
species reactivity   wide range
application(s)   indirect ELISA: 1:10,000 using in situ prepared L-glutamate-glutaraldehyde-BSA conjugate for coating
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

L-glutamic acid (Glu).

Specificity

The antibody reacts specifically with L-glutamate (L-glutamic acid), when immobilized with glutaraldehyde. Cross-reaction is observed with Gly-Glu and Asp-Glu. When using the product at the recommended working dilution, in indirect and competitive ELISA, weak cross-reaction may be obtained with D-glutamate, L-glutamine, L-aspartate, D-aspartate, L-asparagine, β-alanine, glycine, 5-aminovaleric acid, γ-aminobutyric acid (GABA) and Gly-Asp. Glutamate is found in cell bodies, axons, and terminals of glutamatergic neurons in the CNS.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immuno-electron microscopy (1 paper)

Monoclonal Anti-Glutamate antibody produced in mouse is suitable for:
• immunolabeling of glutamate in the lamina of the Drosophila visual system
• immunocytochemistry at a working dilution of 1:20,000 to detect glutamate using heart and extrinsic nerves of stomatopod crustacean, Squilla oratoria
• immunofluorescent staining as a primary antibody at a working dilution of 1:1000 using cryosections of brains and embryos from mice
• use as a primary antibody to measure the glutamate release in synaptosome preparations
It is also suitable for indirect ELISA at a working dilution of 1:10,000 using in situ prepared L-glutamate-glutaraldehyde-BSA conjugate for coating.

Biochem/physiol Actions

Glutamate is an amino acid that acts as a excitatory neurotransmitter in the vertebrate brain and functions in all brain processes. It is released from one end of the neuron and it binds to receptors on the surface of adjacent neurons. Glutamate is released in a calcium-dependent manner in response to depolarizing stimuli. It serves as a substrate in certain metabolic pathways, such as, synthesis of glutamine and γ-aminobutyric acid (GABA) Excessive release of glutamate or aspartate have been observed in the pathogenesis of certain neurodegenerative diseases, such as, Huntington’s disease, Alzheimer’s disease. The same is also observed in selective neuronal degenerations involved in epilepsy, ischemia, and hypoglycemia.

Price and Availability


Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?