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G9637 Sigma

Glycerol 3-phosphate Oxidase from Pediococcus sp.

lyophilized powder, 40-80 units/mg solid, pH 8.1

Synonym: sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase, L-Glycerol 3-phosphate Oxidase

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Description

Application

This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) and glycerokinase (GYK-301, GYK-311) in clinical analysis.

Packaging

100 units in glass bottle

Unit Definition

One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H2O2 per min at 37°C, at the appropriate pH.

Physical form

Lyophilized powder containing stabilizers

Physical properties

Isoelectric point : 4.1 -/+0.1
Michaelis constants : 3.2x10 -3M (L-α-Glycerophosphate), 6.8 x 10 -3M (D, L-form)
Inhibitors : Ionic detergents (SDS, LBS, etc.), Hg++, Ag+
Optimum pH : 8.0 - 8.5Optimum temperature : 35 - 40oC
pH Stability : pH 6.5 - 8.5 (25oC, 20hr) Thermal stability : below 40oC (pH 7.0, 15min)
Substrate specificity : The enzyme has the highest specificity for L-form of a-γlycerophosphate

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Assay Procedure for Glycerol 3-phosphate Oxidase

One unit causes the formation of one micromole of hydrogen peroxide (half a micromole of quinoneimine dye) per minute under the conditions described below.
Keywords: Atomic absorption spectroscopy, Extinction coefficient

Enzymatic Assay of Lipase Type XIII from Pseudomonas Species and Pseudomonas Cepacia using a coupled enzyme system of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

To standardize a procedure for the enzymatic assay of Lipase Type XIII from Pseudomonas Species, Sigma Product Number L9518 , and Pseudomonas Cepacia, Sigma Product Number L9156, using a coupled enzy...
Keywords: Centrifugation, Extinction coefficient, High performance liquid chromatography

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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