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G9785 Sigma

GSEM Supplement

50 ×, liquid, sterile-filtered, suitable for cell culture

Synonym: Glutamine synthetase expression medium supplement

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Properties

Related Categories CHO Platform, Cell Culture, Serum-free Media, Supplements
sterility   sterile-filtered
form   liquid
concentration   50 ×
impurities   endotoxin, tested
suitability   suitable for cell culture
shipped in   dry ice
storage temp.   −20°C

Description

Application

The glutamine synthetase (GS) system is used to express recombinant proteins from mammalian cells. Glutamine-dependent cells transfected with a glutamine synthetase gene can be selectively grown in a suitable glutamine-deficient medium.

Packaging

Contains (mg/L): L-alanine 450.0, L-asparagine · H2O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0

Quantity

One mL will supplement 50 mL of cell culture medium.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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Description

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S4500 L-Serine, ReagentPlus®, ≥99% (HPLC)
S4311 L-Serine, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%
A4036 Adenosine, suitable for cell culture, BioReagent
06693 Timestrip Plus -20 °C
U3003 Uridine, powder, BioReagent, suitable for cell culture
T1895 Thymidine, powder, BioReagent, suitable for cell culture
C4654 Cytidine, BioReagent, suitable for cell culture, powder, ≥99%
G8790 Glycine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, ≥98.5%
G6264 Guanosine, suitable for cell culture, BioReagent

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