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GAGO20 Sigma

Glucose (GO) Assay Kit

sufficient for 20 assays

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Description

Frequently Asked Questions

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Application

This kit is for the quantitative, enzymatic determination of glucose in food and other materials. Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product. The intensity of the pink color measured at 540 nm is proportional to the original glucose concentration.

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Amplified Detection

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Standard component

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o-dianisidine reagent 1 mL/vial SDS    
glucose oxidase/peroxidase reagent 1 capsule SDS    
glucose standard .5 mL SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Enzymatic Food Analysis

Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. Sigma-Aldrich offers a wide...
Jvo Siegrist
Product Manager Microbiology ivo.siegrist@sial.com, AnalytiX Volume 9 Article 2
Keywords: Absorption, Clinical, Degradations, Diabetes, Enzyme activity, Food Analysis, Nutrition, Obesity, Sample preparations

NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field. This technique is non-destructive and can provide full structural info...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Cancer, Digestions, Electrophoresis, Nuclear magnetic resonance spectroscopy, Separation, Spectroscopy

Peer-Reviewed Papers
15

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