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GAGO20 Sigma

Glucose (GO) Assay Kit

sufficient for 20 assays

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

This kit is for the quantitative, enzymatic determination of glucose in food and other materials. Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product. The intensity of the pink color measured at 540 nm is proportional to the original glucose concentration.

Price and Availability


Amplified Detection

All labs need water

Standard component

Description

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o-dianisidine reagent 1 mL/vial SDS    
glucose oxidase/peroxidase reagent 1 capsule SDS    
glucose standard .5 mL SDS    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport

Frequently Asked Questions

Can Product GAGO20, Glucose (GO) Assay Kit, be tested on blood or plasma glucose?
For analysis of such samples, this kit should be useful on samples that have been deproteinized. 
Can Product GAGO20, Glucose (GO) Assay Kit, be used in a 96-well plate format?
Sigma Aldrich has not performed this in a 96-well plate format.  However if a method has have not tried this. If you have developed a method in a 96-well format, Sigma Aldrich would be happy to know about this.
Can frozen samples be tested with Product GAGO20, Glucose (GO) Assay Kit?
One publication has successfully reported use of the GAGO20 on samples frozen at -20 °C.  Further information can be found about this answer by clicking:  http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=412111
Can Product GAGO20, Glucose (GO) Assay Kit, be used on culture media samples?
Sigma Aldrich has not tested this product on culture media samples, but several publications in the literature report successful use of the kit with culture media samples.
What is the difference between the GAGO20 and GAHK20 Glucose (GO) Assay Kits?
The kits use different enzymes. GAGO20 uses glucose oxidase and peroxidase, while GAHK20 uses hexokinase.  Furthermore, the final reaction products are monitored at different wavelengths, 540 nm (GAGO20) and 340 nm (GAHK20).
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
How would I decolorize my sample for use in the glucose assay using Product GAGO20, Glucose (GO) Assay Kit?
First, you will need to determine if the color affects the result by reading it as a blank in the assay. The classical method for decolorization of carbohydrate solutions is treatment with charcoal. A small amount of powdered charcoal such as Product C3345 is added to the sample, stirred a few moments, then clarified by filtration or centrifugation. If the color persists, another portion of charcoal is added and the process repeated until there is no more change in color with an addition of charcoal.
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Protocols & Articles

Articles

Enzymatic Food Analysis

Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. Sigma-Aldrich offers a wide...
Jvo Siegrist
Product Manager Microbiology ivo.siegrist@sial.com, AnalytiX Volume 9 Article 2
Keywords: Absorption, Clinical, Degradations, Diabetes, Enzyme activity, Food Analysis, Nutrition, Obesity, Sample preparations

NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field. This technique is non-destructive and can provide full structural info...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Cancer, Digestions, Electrophoresis, Nuclear magnetic resonance spectroscopy, Separation, Spectroscopy

Peer-Reviewed Papers
15

References

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