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GAHK20 Sigma

Glucose (HK) Assay Kit

sufficient for 20 assays

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

This kit is for the quantitative, enzymatic determination of glucose in food and other material. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phospho-gluconate in the presence of oxidized nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to glucose concentration.

Price and Availability


Amplified Detection

All labs need water

Standard component

Description

Product #

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glucose (HK) assay reagent 20 mL SDS    
glucose standard .5 mL SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Frequently Asked Questions

How do I determine what sample size to use in the Glucose (HK) Assay Kit, Product GAHK20?
If you know approximately how much glucose is present, dilute the sample down to 0.5 to 50 ug of glucose and use between 10 to 200 ul sample volume.  If you do not know how much glucose is present, you should assay the undiluted sample and dilutions of it.  Based on the dilution factor you can determine the glucose concentration.  To view this information on the technical bulletin please click on the following link: http://www.sigmaaldrich.com/sigma/bulletin/gahk20bul.pdf
Can the procedure for Glucose (HK) Assay Kit, Product GAHK20, be scaled down?
Although we have not done it, you should be able to scale down the procedure as long as you have pipettes capable of dispensing accurate ul volume quantities and an instrument that can monitor the 340 nm wavelength.
If my sample for the Glucose (HK) Assay Kit, Product GAHK20, has a lot of protein, how do I deproteinize it?
You can deproteinize by using barium hydroxide, B4059, and zinc sulfate, Z2876, by adding 0.2 ml of sample to 1.8 ml of water, swirl and mix, then 1.0 ml of barium hydroxide, swirl and mix, then 1.0 ml of zinc sulfate, swirl and mix thoroughly by shaking, and then filter or centrifuge to obtain clear filtrate.
How do I decolorize a solution for use with Product GAHK20, Glucose (HK) Assay Kit?
You can decolorize a solution by adding a small amount of charcoal to the solution, swirl, then filter or centrifuge to remove charcoal.  To view this information on the Product Information Sheet for charcoal, C3345, please click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/Product_Information_Sheet/c3345pis.Par.0001.File.tmp/c3345pis.pdf
How do I degas a solution for use with Product GAHK20, Glucose (HK) Assay Kit?
You can degas a solution by either putting it under a vacuum or by bubbling nitrogen through it.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

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Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. Sigma-Aldrich offers a wide...
Jvo Siegrist
Product Manager Microbiology ivo.siegrist@sial.com, AnalytiX Volume 9 Article 2
Keywords: Absorption, Clinical, Degradations, Diabetes, Enzyme activity, Food Analysis, Nutrition, Obesity, Sample preparations

NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field. This technique is non-destructive and can provide full structural info...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Cancer, Digestions, Electrophoresis, Nuclear magnetic resonance spectroscopy, Separation, Spectroscopy

Peer-Reviewed Papers
15

References

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