• USA Home
  • GRSA - Glutathione Reductase Assay Kit

EMAIL THIS PAGE TO A FRIEND
GRSA Sigma

Glutathione Reductase Assay Kit

Purchase

Properties

Related Categories Cell Analysis, Cell Biology, Cell Signaling and Neuroscience, Cell Stress, Nitric Oxide and Cell Stress,
usage   sufficient for 100 tests
storage temp.   2-8°C
Gene Information   human ... GSR(2936)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

UV assay: One unit will cause the oxidation of 1.0 μmole of NADPH at 25 °C at pH 7.5.
Colorimetric assay: One unit will cause the reduction of 1.0 μmole of DTNB to TNB at 25 °C at pH 7.5.

Biochem/physiol Actions

Glutathione reductase (EC 1.6.4.2) (GR) is a ubiquitous enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). Glutathione reductase is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also a substrate for the glutathione peroxidases and glutathione S-transferases in the detoxification of organic peroxides and the metabolism of xenobiotics. This kit contains reagents for the spectrophotometric determination of glutathione reductase activity either by following the decrease in absorbance caused by the oxidation of NADPH at 340 nm (UV assay) or the increase in absorption caused by the reduction of dithiobis(2-nitrobenzoic acid) (DTNB) at 412 nm (colorimetric assay). This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid).

This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid).

Price and Availability


Flow Cytometry Instruments

Kit component only

Description

Product #

Add to Cart

Glutathione Reductase Assay Buffer 125 mL SDS    
Glutathione Reductase Dilution Buffer 100 mL SDS    
Glutathione Reductase Positive Control 1 mL/vial SDS    

Standard component

Description

Product #

Add to Cart

5,5′-Dithiobis(2-nitrobenzoic acid) 50 mg SDS D8130
Glutathione, oxidized, disodium salt 100 mg SDS G4626
β-Nicotinamide adenine dinucleotide 25 mg SDS N6505
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

G9297 Glutathione Reductase human, buffered aqueous solution, ≥10 units/mg protein, recombinant, expressed in E. coli
08168 Timestrip Plus 8 °C
38185 Quantification kit for oxidized and reduced glutathione, 200 tests

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?