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GUSS Sigma

β-Glucuronidase Reporter Gene Staining Kit

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Description

Application

The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.

Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.

Features and Benefits

• Histochemical staining of plant tissues expressing the E. coli GUS enzyme
• Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
• GUS does not interfere with plant cell function or viability

Other Notes

The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.

General description

Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.

Price and Availability


Amplified Detection

Biomedical Applications

Kit component only

Description

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2× Fixation buffer 25 mL SDS    
Reagent A 50 mL SDS    
Reagent B 200 μL SDS    
Reagent C 200 μL SDS    

Standard component

Description

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X-GlcA 1 mL/vial SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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