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H0273 Sigma

Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse

clone IAP-28, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Chaperonins and Heat Shock Proteins,
species reactivity   guinea pig, mouse, frog, rabbit, chicken, bovine, rat, human, turkey, Drosophila
application(s)   immunohistochemistry: suitable using frozen sections of chicken gizzard smooth muscle
  indirect ELISA: suitable
  indirect immunofluorescence: suitable using formalin-fixed, heat-shocked cultured chick embryo fibroblasts
  radioimmunoassay: suitable
  western blot: 1:500 using turkey gizzard extract
clone   IAP-28, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 25 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HSPB1(3315)
mouse ... Hspb1(15507)
rat ... Hspb1(24471)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle.

Specificity

The antibody also reacts with a dimer of the HSP 25 (approx. 58 kDa) appearing in preparations stored for prolonged periods prior to electrophoretic analysis. A major proteolytic fragment of 22 kDa may also be detected. The antibody suppresses the inhibition of actin elongation induced by the 25 kDa IAP. The antibody displays a punctate or fine fibrillary pattern of labeling throughout the cytoplasm in heat-shocked cells and co-localization with mitochondria. Reactivity has been observed with HSP 25 of various organs.

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