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H0396 Sigma

Hydrocortisone-Water Soluble

BioReagent, suitable for cell culture

Synonym: Cyclodextrin-encapsulated hydrocortisone



Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Corticosteroid, Cytokines, Growth Factors and Hormones,
product line   BioReagent
solubility   H2O: soluble400 mg/mL
suitability   suitable for cell culture
Gene Information   human ... ABCB1(5243), CYP3A4(1576), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)


Frequently Asked Questions

Frequently Asked Questions are available for this Product.

General description

Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.


100 mg in poly bottle

Package size based on hydrocortisone

Price and Availability

All labs need water
Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
How do I calculate the molarity of product H0396, Hydrocortisone-Water Soluble?
Product H0396 (Hydrocortisone-Water Soluble) is a complex of Hydrocortisone and cyclodextrin. Cyclodextrin is used to aid in the solubility of Hydrocortisone into water. In order to prepare a molar solution of Hydrocortisone you will have to base it on the Hydrocortisone content in the solution and not the entire complex.  Product H0396 is formulated to contain approximately 85 mg Hydrocortisone per gram of powder.  Based on this average, if you are preparing a 400 mg/ml stock solution, there will be approximately 34 mg of Hydrocortisone in 1 ml.  Below is the calculated molarity of Hydrocortisone when the complex is dissolved at 400 mg/ml in water.  molecular weight X unknown molarity X volume = complex weight X complex ratio 362.46 g/mol X Molarity X 1 ml = 400 mg X 0.085        Molarity = 0.094 M Hydrocortisone molecular weight = 362.46 g/mol molarity (unknown) = mol/L volume = 1ml complex weight = 400 mg complex ratio = 85 mg Hydrocortisone/1 gm complex
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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C0926 (2-Hydroxypropyl)-β-cyclodextrin, powder, BioReagent, suitable for cell culture

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