HIS-Select^® HF Nickel Affinity Gel

Application

Affinity gel is suitable for the purification of histidine tagged proteins in native and denaturing conditions.

Nickel Affinity Gel for the purication of histidine tagged proteins. HIS-Select™ High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC^™ applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.

Features and Benefits

• High selectivity for higher purity
• Unique non-charged hydrophilic linkage reduced non-specific binding
• Binding Capacity for histidine-tagged protein is greater than 15 mg/ml
• Highly cross-linked agarose matrix allows for purification under higher pressures
• Binding under denaturing or non-denaturing conditions
• One-step purification

General description

HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind histidine containing proteins in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.

Preparation Note

Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.

Physical form

50% suspension in 30% ethanol

Legal Information

FPLC is a trademark of GE Healthcare

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC