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H0537 Sigma

HIS-Select® HF Nickel Affinity Gel

Properties

Related Categories HIS-Select, HIS-Select Affinity Purification, HIS-Select Convenient Formats, HIS-Select Resins, His,
matrix   Highly cross-linked 6% Beaded Agarose
capacity   15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)
storage temp.   2-8°C

Description

Application

Affinity gel is suitable for the purification of histidine tagged proteins in native and denaturing conditions.

Nickel Affinity Gel for the purication of histidine tagged proteins. HIS-Select™ High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.

Features and Benefits

• High selectivity for higher purity
• Unique non-charged hydrophilic linkage reduced non-specific binding
• Binding Capacity for histidine-tagged protein is greater than 15 mg/ml
• Highly cross-linked agarose matrix allows for purification under higher pressures
• Binding under denaturing or non-denaturing conditions
• One-step purification

General description

HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind histidine containing proteins in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.

Preparation Note

Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.

Physical form

50% suspension in 30% ethanol

Legal Information

FPLC is a trademark of GE Healthcare

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC

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