|Related Categories||HIS-Select, HIS-Select Affinity Purification, HIS-Select Convenient Formats, HIS-Select Resins, His,|
|matrix||Highly cross-linked 6% Beaded Agarose|
|capacity||15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)|
Affinity gel is suitable for the purification of histidine tagged proteins in native and denaturing conditions.
Nickel Affinity Gel for the purication of histidine tagged proteins. HIS-Select™ High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC™ applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.
• High selectivity for higher purity
• Unique non-charged hydrophilic linkage reduced non-specific binding
• Binding Capacity for histidine-tagged protein is greater than 15 mg/ml
• Highly cross-linked agarose matrix allows for purification under higher pressures
• Binding under denaturing or non-denaturing conditions
• One-step purification
HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind histidine containing proteins in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.
Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.
50% suspension in 30% ethanol
FPLC is a trademark of GE Healthcare
HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC
Certificate of Analysis
Certificate of Origin
|Symbol||GHS02, GHS07, GHS08, GHS09|
|Hazard statements||H226-H302 + H332-H315-H317-H334-H341-H350i-H360D-H371-H372-H411|
|Precautionary statements||P201-P260-P273-P280-P308 + P313|
|Personal Protective Equipment||Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter|
|Hazard Codes (Europe)||T,N|
|Risk Statements (Europe)||49-61-10-20/21/22-38-42/43-48/23-51/53-68-68/20/21/22|
|Safety Statements (Europe)||53-23-36/37-45-61|
|RIDADR||UN 1170 3/PG 3|
|Flash Point(F)||87.8 °F|
|Flash Point(C)||31 °C|
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