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H0887 Sigma

HEPES solution

1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Synonym: N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions, Buffers A to Z,
InChI Key   JKMHFZQWWAIEOD-UHFFFAOYSA-N
Quality Level   PREMIUM
sterility   sterile-filtered
product line   BioReagent
form   liquid
concentration   1 M
impurities   endotoxin, tested
pH   7.0-7.6
useful pH range   6.8 - 8.2
pH range   7.0 - 7.6
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Caution

Concentrations of HEPES over 40mM may be toxic to some cell types.

Preparation Note

Prepared from HEPES free acid and cell culture grade water.

Application

HEPES is an organic zwitterionic buffering agent effective in the physiological pH range of 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. This product has been qualified for use in cell culture.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

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