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H1029 Sigma

Monoclonal Anti-polyHistidine antibody produced in mouse

clone HIS-1, ascites fluid

Synonym: Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal Anti-polyHistidine, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, HF-HI,
biological source   mouse
antibody form   ascites fluid
clone   HIS-1, monoclonal
form   (Suppled as ascites fluid with 15mM sodium azide.)
application(s)   dot blot: suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotype   IgG2a
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Antibody suitable for immunoblotting, dot blot assays, ELISA and immunocytochemistry.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Immunogen

recombinant polyHistidine tagged fusion protein.

Other Notes

Recommended dilution for immunoblotting is 1:3000

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

Specificity

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

General description

The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors pET, pRSET, and pTrc.

Price and Availability


Tools for Large Molecule BioAnalysis

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Peer-Reviewed Papers
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