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  • H1029 - Monoclonal Anti-polyHistidine antibody produced in mouse

H1029 Sigma

Monoclonal Anti-polyHistidine antibody produced in mouse

clone HIS-1, ascites fluid

Synonym: Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal Anti-polyHistidine, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag



Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, HF-HI,
biological source   mouse
antibody form   ascites fluid
clone   HIS-1, monoclonal
form   (Suppled as ascites fluid with 15mM sodium azide.)
application(s)   dot blot: suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotype   IgG2a
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


Antibody suitable for immunoblotting, dot blot assays, ELISA and immunocytochemistry.

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recombinant polyHistidine tagged fusion protein.

Other Notes

Recommended dilution for immunoblotting is 1:3000

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.


The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

General description

The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors pET, pRSET, and pTrc.

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Safety Information

NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
How many histidines are required for proper recognition by Product H1029, Monoclonal Anti-polyHistidine antibody?
Product H1029, Monoclonal Anti-polyHistidine antibody requires at least 6 histidines in a row for recognition.  More information can be found at H1029's Product Information Sheet (link provided below).     Link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/2/h1029dat.Par.0001.File.tmp/h1029dat.pdf.
What is the detection limit of Product H1029, Monoclonal Anti-polyHistidine antibody?
The detection limit of product H1029 is 0.5 μg/ml or less.  More information can be found at H1029's Product Information Sheet (link provided below).      Link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/2/h1029dat.Par.0001.File.tmp/h1029dat.pdf.
Is Product H1029, Monoclonal Anti-polyHistidine antibody, suitable for use in immunofluorescence studies?
Product H1029 has been used in immunofluorescence and a good picture of the staining has been published. Ker R. Marshall, et al., Journal of General Virology 83, 1601-1612 (2002).
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